Morphopatological investigations in experimental intoxication with Deoxynivalenol in mice. (Summary).

Publication Type

Contribution to conference

Authors

Macri, A., Baba, A.I., Dancea, Z., Catoi, C., Schollenberger, M., Drochner, W.

Year of publication

2004

Published in

"Bulletin of the University of Agricultural Sciences and Veterinary Medicine"

Editor

Univ. of Agricultural Sciences and Veterinary Medicine

Pubisher

AcademicPres , Cluj-Napoca (Rumänien)

Page (from - to)

279

Conference name

Prospects of the 3rd Millenium Agriculture.

Conference location

Cluj-Napoca

Conference date

20.-23.10.2004

Keywords

Deoxynivalenol, mice, mycotoxin

Deoxynivalenol (DON, vomitoxin) is a trichotecene mycotoxin produced by Fusarium species and prevalent world-wide in all sorts of cereal grains (l). In animal models, DON causes feed refusal, decreased weight gain, cardiotoxicity and teratogenity(2). DON has also been shown to affect cell mediated and humoral immunity in several animal species(3). The paper is the result of research on three experimental groups and a control group. Group 1, composed of four mice injected i.p. with 7.5 mg DON/kg, slaughtered a week later; group 2 composed of four mice inoculated i.p. with 7.5 mg DON/kg, slaughtered two weeks later; group 3 composed of four mice inoculated i.p. with 25 mg DON/kg, slaughtered two weeks later; group 4 control, composed of ten mice inoculated i.p. with physiological salt solution with a dose of 25 ml/mouse. Within group 1 the reactive modifications of a regenerative type have been noticed at the level of the majority of the examined organs and structures. In the intestine, young cells, an obvious hypertrophy and a big number of gastric parietal cells and an active oesophagogastric epithelium, slightly thickned and an abundant parakeratosic stratum have been noticed. In the liver hypertrophied hepatocites, with big nuclei or with two or three nuclei. In the renal glomerules a hypertrophic reaction of cellularization with podocites and a mezangiale cells is noticed. The lymphoid follicles in the cortical area are populated with cells of lymphohistioid type, with abundant cytoplasm, with big heterogenous nuclei, which proves a regenerative activity. In the case of group 2 it can be asserted that the examined structures do not present lesions which could be attributed to vomitoxin, except mesenteric lymph nodes in which there are still alterative images of a necrotic and apoptotic type. On the other hand regenerative images appear in the intestine and liver. Within group 3 it can be asserted that the mice remake their main organs (intestine, liver, stomach,